I´ve tried to ligate a 6kb-fragment into a Gal4-Transformation-Vektor
(p221-4; 12kb long), but failed several times. Does anybody have a
protocol or reference for such a cloning problem ?? I hope, it´s not
impossible
Thanks
--
Arnd Richardt
Arndt.Richardt at ruhr-uni-bochum.de
Ruhr-Universität Bochum
Fakultät Chemie
Molekulare Zellbiochemie
Gebäude NC 5 / 171
44780 Bochum
