N-acetyl transferase purification, What to do next?

lnd at mail.utexas.edu lnd at mail.utexas.edu
Sat Mar 6 13:39:19 EST 1999

If your protein is well soluble in water, I mean if that's a cytosolic one,
so why do not you run that 2-prot fraction on gradient SDS-Gel and cut band
for further in gel digestion/peptide mapping or sequenscing?

You can, once, extract all bands separately and test enzymic activity...so
later will know which one should be digested.


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