Hi Netters
Can someone tell me which HPLC method/column in the best one to separate
highly glycosolated membrane proteins with GPI-anchore? I tried
RP-HPLC, but 2 major proteins tend to migrate together. I need to
separate them for further digestion and peptide mapping.
Also, those proteins are easily separated by SDS-PAGE (63 and 23 kDa) -
so I am interested which columns would be the best option to separate
them based on mol.mass?
Any ideas?
Thanx
L.D.
