Hi Proteiner
Since the two components have two very different MWs, it should be better to
use a gel filtration column. They are usually called SPHEROGEL PWHR from
Beckman. They will be separated by a few minutes.
Good luck
Mahmood
lnd at mail.utexas.edu wrote:
> Hi Netters
>> Can someone tell me which HPLC method/column in the best one to separate
> highly glycosolated membrane proteins with GPI-anchore? I tried
> RP-HPLC, but 2 major proteins tend to migrate together. I need to
> separate them for further digestion and peptide mapping.
>> Also, those proteins are easily separated by SDS-PAGE (63 and 23 kDa) -
> so I am interested which columns would be the best option to separate
> them based on mol.mass?
>> Any ideas?
>> Thanx
>> L.D.
