How to handle PAS?

Rich Dudley rdudley+ at pitt.edu
Thu Mar 4 09:44:11 EST 1999

Wiebke Schmidt wrote:

> I want to compare the activities of several mutants of an enzyme. In the
> assay immunoprecitates of the enzyme are used. I have problems in using
> equal amounts of immunoprecipitate in each assay because of the
> consistence of the protein A-Sepharose (PAS). Does anyone have a
> suggestion how to pipette  equal amounts of PAS?
> Thanks in advance,
> Wiebke

I cut the end off of a 200 ul tip and use that.  Works fairly well.


--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
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