In article <7kpofb$ec5$1 at dailyplanet.wam.umd.edu>, plato at wam.umd.edu (SNL) wrote:
:I'm looking for information on how to attach a protein of interest that
:binds DNA onto a column, and using the column to purify specific DNA
:sequences.
::Any references appreciated. Anyone know of a kit that can attach protein
:to some sort of bead to make a column?
There are many ways of doing this. Check Sigma, Bio-Rad,
Pharmacia catalogs, "pre-activated sorbents" sections. Also, check
your nearest library for books on "affinity chromatography". It really is
simple: You buy appropriate sorbent and add to it protein solution of
appropriate concentration in appropriate buffer. What is "appropriate"
depends on your exact application and other variables.
- Dima
