Bob wrote:
> Is there any web site that would calculate the absorption coefficient of a
> protein on the basis of the number of tryptophans, tyrosins, Phe, disulfide
> bonds...
>> Or if not, what is the formula?
>> Thanks, Rob
Hi, Rob,
Try a good paper by Pace et al. (1995) in Protein Science 4,2411-2423. But pay
attention that there are no any method to exactly calculate an extinction
coefficient. A calculated value can be used for estimation only (however the
estimation may be quite good!). I'd recommend to measure (i) using Biuret or
other absolute method of the protein concentration determination and (ii)
applying a procedure for light scattering subtraction at the absorption spectra
determination.
Best wishes
Eugene
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