Silke Beismann <sbeisma at uni-molgen.gwdg.de> wrote:
> during my protein purification I have a lot of trouble to get rid of
> nucleic acids. I have to use high amounts of Benzonase, dialyse
> extensively, use several chromatography columns and so on. Now I read in
> a paper a protocoll for streptomycin sulfate precipitation of nucleic
> acids. Has anyone used this method before? Is it mild to the protein and
> efficiently in respect of the nucleic acids?
Our lab used this when purifying recombinant phosducin from E.coli
before applying the supernatant to a MonoQ column. The reference is
probably either
Bauer PH, Müller, Puzicha M, Pippig S, Obermaier B, Helmreich EJM,
Lohse MJ: Phosducin is a protein kinase A-regulated G-protein
regulator. Nature 358: 73-76 (1992).
or
Hekman M, Bauer PH, Söhlemann P, Lohse MJ: Phosducin inhibits
receptor phosphorylation by the beta-adrenergic receptor kinase in
a PKA-regulated manner. FEBS Lett. 343: 120-124 (1994).
However, since then the lab has switched to using his-tagged proteins,
simply because the protein purification is much more convenient (no HPLC
needed for same purity).
Hope that helps,
--Cornelius.
--
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany email: phak004 at rzbox.uni-wuerzburg.de SP4 */
/* "Science is the game we play with God to find out what His rules are." */
