On Wed, 30 Jun 1999, Peter Prevelige wrote:
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>> Far UV CD spectra should be calculated in terms of deg cm2 dmol/ AMINO
> ACID. In this manner, tey are independent of the protein concentration,
> as well as the molecular weight of the protein. Therefore, there is no
> concentration dependent change in helicity, unless there is molecular
> association going on.
>>>> Young Chai wrote:
> >
> > Could you tell me that there are any differences of the percentage of
> > alpha helix from CD spectra with different protein concentration?
> > I appreciate kind answers.
> >
> > Young Chai
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>>>But a CD spectrum intensity does depend on the protein concentration. So
to calculate a secondary structure from CD, the protein concentration is
necessary while the sendary structure itself is concetration-independent
as Prof.Prevelige noted correctly.
Best wishes.
Dr.Eugene Gussakovsky
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