revers zymography

Bjørn Olav Kvamme Bjorn.Kvamme at mbi.uib.no
Fri Jan 29 04:48:08 EST 1999


I'm characterising protease inhibitors from a crude plantprotein
solution. One of the techniques I want to try is to run revers
zymography with a copolymerized substrate like gelatin or casein, and
trypsin or chymotrypsin as proteolytic enzym.

I'f any of you have a good protocol/refference for this, I would
appreciate it if you notified me about it.

Bjørn Olav
Bjorn.Kvamme at mbi.uib.no

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