IUBio

A problem with SDS

Wolf-Dieter Stalz stalz at biologie.uni-osnabrueck.de
Fri Jan 29 08:39:15 EST 1999



Roberto Calcedo wrote:

> Dear all:
>
> I have a protein sample that has SDS bound to it.
>
> Anybody know how could I take away the SDS from protein sample?
>
>       Thanks

Hi Roberto,
we tried Ion-pair Extraction on SDS denaturated proteins according to
the protocol described in Analytical Biochemistry (1979) 93 153-157 by
Henderson at al.
Seems as if it works in some cases.
Wolf-Dieter
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