Membrane protein isolation for western blotting

Peter pxpst2 at unixs.cis.pitt.edu
Mon Jan 25 10:15:15 EST 1999

In article <78gq9q$9je$1 at panix3.panix.com>, iayork at panix.com (Ian A. York)

> Disrupt the cells mechanically (traditionally douncing works) and
> centrifuge first at low speed (to get rid of nuclei, if you want to do so)
> and then at around 100,00g (if I recall correctly) for an hour or so.  The
> membranes pellet.

This is DEFINITELY the easiest way to to enrich for membranes.  


" Don't you eat that yellow snow
     Watch out where the huskies go"

More information about the Proteins mailing list

Send comments to us at biosci-help [At] net.bio.net