Membrane protein isolation for western blotting

Amanda Nouwens anouwens at RNA.BIO.MQ.EDU.AU
Sun Jan 24 23:28:51 EST 1999

To:             	protein-analysis at net.bio.net
From:           	Neal Melvin <nrmelvin at acs.ucalgary.ca>
Subject:        	Membrane protein isolation for western blotting
Date sent:      	Sun, 24 Jan 1999 20:19:45 -0700

> Is there a way to selectively isolate/precipitate membrane proteins
> prior to western blotting??

Check out the paper by Molloy et a, 1998 in Electrophoresis 19:837-844  
(Extraction  of membrane proteins by differential solubilization for separation  
using two-dimensional gel electrophoresis). 

The method works very well, and you could use it (or modify) for standard 1D  
gels also.  



Amanda Nouwens
Australian Proteome Analysis Facility (APAF)
Macquarie University
Sydney, NSW 2109
Tel: 02 9850 6209
Fax: 02 9850 6200
Email: anouwens at proteome.org.au

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