To: protein-analysis at net.bio.net
From: Neal Melvin <nrmelvin at acs.ucalgary.ca>
Subject: Membrane protein isolation for western blotting
Date sent: Sun, 24 Jan 1999 20:19:45 -0700
> Is there a way to selectively isolate/precipitate membrane proteins
> prior to western blotting??
>
Check out the paper by Molloy et a, 1998 in Electrophoresis 19:837-844
(Extraction of membrane proteins by differential solubilization for separation
using two-dimensional gel electrophoresis).
The method works very well, and you could use it (or modify) for standard 1D
gels also.
Cheers,
Amanda.
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Amanda Nouwens
Australian Proteome Analysis Facility (APAF)
Macquarie University
Sydney, NSW 2109
Tel: 02 9850 6209
Fax: 02 9850 6200
Email: anouwens at proteome.org.au
