Membrane protein isolation for western blotting

Ian A. York iayork at panix.com
Sun Jan 24 23:00:58 EST 1999

In article <78gnsu$331a at ds2.acs.ucalgary.ca>,
Neal Melvin  <nrmelvin at acs.ucalgary.ca> wrote:
>Is there a way to selectively isolate/precipitate membrane proteins
>prior to western blotting??

Disrupt the cells mechanically (traditionally douncing works) and
centrifuge first at low speed (to get rid of nuclei, if you want to do so)
and then at around 100,00g (if I recall correctly) for an hour or so.  The
membranes pellet.


    Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England

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