A problem with SDS

docstymie at yahoo.com docstymie at yahoo.com
Fri Jan 22 20:29:55 EST 1999

In article <pxpst2-2001990919420001 at pelli.pathology.pitt.edu>,
  pxpst2 at unixs.cis.pitt.edu (Peter) wrote:
> In article <oibcader-1801991534170001 at lcmx83.lc.ehu.es>,
> oibcader at lg.ehu.es (Roberto Calcedo) wrote:
> > Dear all:
> >
> > I have a protein sample that has SDS bound to it.
> >
> > Anybody know how could I take away the SDS from protein sample?
> Dialysis would be a good first step, but the removal of all the SDS is
> difficult.

Gellman has a way of refolding proteins that have been denatured by SDS using
cyclodextrins to strip away the SDS from the protein. I don't know if the
cyclodextrins would work with a folded protein.


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