I am surprised you removed ALL SDS. I have tried ion-exchange chromatography
in the past and it did not work.
Your protein, is it an enzyme? If not, how do you now it's healthy?
Achim
Peter Hains wrote in message <788rht$m9q$2 at sunb.ocs.mq.edu.au>...
>Time for my two bobs worth,
>>I recommend using a mixed bed resin such as BioRads AG11A8. I have
>successfully used this to remove SDA from my proteins. All you need is a
>simple column and your protein comes out healthy.
>>Peter.
>>>>>I'm afraid I don't have a clever saying to put here.
>>Remove NOSPAM to e-mail me.
>Peter Hains Ph. +61 2 9850 6216
>Australian Proteome Analysis Facility Fax. +61 2 9850 6200
>Level 4 Building F7B
>Macquarie University, Sydney 2109
>>>>
