Prestained MW standards for SDS PAGE

Peter pxpst2 at unixs.cis.pitt.edu
Thu Jan 21 09:24:03 EST 1999

In article <785k2f$2f66 at ds2.acs.ucalgary.ca>, Neal Melvin
<nrmelvin at acs.ucalgary.ca> wrote:

> I'm using pre-stained, broad range MW standards (188-7kd) from Bio-Rad.
> The four
> heaviest markers show up as a nice, tight band in the gel, but the lower
> four are very broad, with poor resolution... is there any way to fix
> this??

Increase the percentage acrylamide (%T).  If you are running standard
Laemili gels of 10 % or less then you should expect your lower molecular
weight stds to run in broadbands.  If you are interested in having all the
bands be sharp, you have several options. 1) Run Tris Tricine gels  2) Run
gradient gels.
Hope this helps

" Don't you eat that yellow snow
     Watch out where the huskies go"

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