A problem with SDS

Achim Recktenwald achimr at home.com
Wed Jan 20 14:19:57 EST 1999

You could also try an acetone-precipitation at -20°C.
Your protein should stay healthy, while the SDS dissolves in the acetone.


"Hiranya S. Roychowdhury" wrote in message
<199901201632.JAA28288 at nestor.NMSU.Edu>...
>At 09:19 AM 1/20/99 -0500, Peter wrote:
>>In article <oibcader-1801991534170001 at lcmx83.lc.ehu.es>,
>>oibcader at lg.ehu.es (Roberto Calcedo) wrote:
>>> Dear all:
>>> I have a protein sample that has SDS bound to it.
>>> Anybody know how could I take away the SDS from protein sample?
>>Dialysis would be a good first step, but the removal of all the SDS is
>>" Don't you eat that yellow snow
>>     Watch out where the huskies go"
>>                                    FZ
>Indeed, once bound, it is difficult to remove SDS from protein by simple
>dialysis. However, dialyzing against 1% Triton X-100 will improve the
>situation. Another option is to electroelute the protein in 0.5%
>triton-containing buffer.
>Dr. Hiranya Sankar Roychowdhury
>New Mexico State University
>Las Cruces, NM 88003
>Ph. (505) 646-5785
>hroychow at nmsu.edu

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