You could also try an acetone-precipitation at -20°C.
Your protein should stay healthy, while the SDS dissolves in the acetone.
Achim
"Hiranya S. Roychowdhury" wrote in message
<199901201632.JAA28288 at nestor.NMSU.Edu>...
>At 09:19 AM 1/20/99 -0500, Peter wrote:
>>In article <oibcader-1801991534170001 at lcmx83.lc.ehu.es>,
>>oibcader at lg.ehu.es (Roberto Calcedo) wrote:
>>>>> Dear all:
>>>>>> I have a protein sample that has SDS bound to it.
>>>>>> Anybody know how could I take away the SDS from protein sample?
>>>>Dialysis would be a good first step, but the removal of all the SDS is
>>difficult.
>>>>Peter
>>>>--
>>" Don't you eat that yellow snow
>> Watch out where the huskies go"
>> FZ
>>>>>>Indeed, once bound, it is difficult to remove SDS from protein by simple
>dialysis. However, dialyzing against 1% Triton X-100 will improve the
>situation. Another option is to electroelute the protein in 0.5%
>triton-containing buffer.
>>>Dr. Hiranya Sankar Roychowdhury
>GENE LAB/ EPPWS
>New Mexico State University
>Las Cruces, NM 88003
>Ph. (505) 646-5785
>hroychow at nmsu.edu>
