A problem with SDS

Hiranya S. Roychowdhury hroychow at NMSU.EDU
Wed Jan 20 11:39:00 EST 1999

At 09:19 AM 1/20/99 -0500, Peter wrote:
>In article <oibcader-1801991534170001 at lcmx83.lc.ehu.es>,
>oibcader at lg.ehu.es (Roberto Calcedo) wrote:
>> Dear all:
>> I have a protein sample that has SDS bound to it.
>> Anybody know how could I take away the SDS from protein sample?
>Dialysis would be a good first step, but the removal of all the SDS is
>" Don't you eat that yellow snow
>     Watch out where the huskies go"
>                                    FZ

Indeed, once bound, it is difficult to remove SDS from protein by simple
dialysis. However, dialyzing against 1% Triton X-100 will improve the
situation. Another option is to electroelute the protein in 0.5%
triton-containing buffer.

Dr. Hiranya Sankar Roychowdhury
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

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