At 09:19 AM 1/20/99 -0500, Peter wrote:
>In article <oibcader-1801991534170001 at lcmx83.lc.ehu.es>,
>oibcader at lg.ehu.es (Roberto Calcedo) wrote:
>>> Dear all:
>>>> I have a protein sample that has SDS bound to it.
>>>> Anybody know how could I take away the SDS from protein sample?
>>Dialysis would be a good first step, but the removal of all the SDS is
>difficult.
>>Peter
>>--
>" Don't you eat that yellow snow
> Watch out where the huskies go"
> FZ
>>
Indeed, once bound, it is difficult to remove SDS from protein by simple
dialysis. However, dialyzing against 1% Triton X-100 will improve the
situation. Another option is to electroelute the protein in 0.5%
triton-containing buffer.
Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu
