pro sequence affect translation?

esb microb at esb.utexas.edu
Mon Jan 18 17:49:24 EST 1999

entire Bovine Pancreatic Trypsin Inhibitor cDNA contains pre sequence (for
secretion) pro sequence (these two are all processed out in mature protein)
and mature sequence.
i tried to express mature BPTI and pro+mature BPTI in e.coli periplasm, and
found out that pro+mature BPTI express much more poorly then mature BPTI
only. i guess the pro sequence has something to do with it. but i could not
see any sign of degradation by western.
so my question is: anyone remember any reference about pro sequence
affecting protein expression?
thanks in advance.

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