two hybrid system

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Thu Jan 14 21:27:39 EST 1999

In article <369DF6CF.14F1B8A4 at ccr.jussieu.fr>, Mathias Brault
<brault at ccr.jussieu.fr> wrote:

> Is it possible to use a two-hybrid system in order to identify cDNA
> encoding protein able to bind a plasma-membrane protein ?
> Thank you
> Mathias Brault

Stratagene is about to release a 2 hybrid kit that relies
on interaction at the cell membrane.  I've seen a couple
of presentations on it and it looks good (although nowhere
near as refined as the classic 2-hybrid).  I don't know
when it is actually due for release but it may be worth
tackling your local rep.
     Bonne chance,
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA

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