IUBio

5 kDa protein detection

piet l at w.v
Tue Jan 12 12:14:07 EST 1999


Georg Lipps wrote in message <3694d730.31953936 at newsserv.uni-bayreuth.de>...
>Hi,
>
>why not try a 16% Tricine-SDS-Gel (Schägger & von Jagow) , works good
>down to at least 2 kDa.
>
>Good luck,
>
>Georg

Yes, works for me. However when staining with CBB, stain for at least one
hour shaking (do not try to heat the gel because the protein then diffuses
out of the gel). Decolor o/n and then you must see the band very clearly.





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