In article <76ss36$rqk$1 at wnnews.sci.kun.nl>, "Gerrit Bouw" <gbouw at sci.kun.nl> wrote:
:Dear everyone,
::Does anyone ever tried to transfect sperm-cells? If so, please could you
:give
:me some guidance on this?
::Gerrit
::
[what I don't unde4rstand is why this has been posted to *.proteins and
not to methods and reagents...]
Yes, spermatozoa are just normal cells and can be lectroporated like any
other. A 1 minute search in Medline:
Title
Sperm as a carrier to introduce an exogenous DNA fragment into the
oocyte ╕
of Japanese abalone (Haliotis divorsicolor suportexta).
╕
Source
╕
Transgenic Research. 6(1):85-95, 1997 Jan.
Title
Nuclear internalization of foreign DNA by zebrafish spermatozoa and
its ╕
enhancement by electroporation.
╕
Source
╕
Journal of Experimental Zoology. 274(2):121-9, 1996 Feb 1.
Title
Sperm characteristics and outcome of human assisted fertilization by
╕
subzonal insemination and intracytoplasmic sperm injection [see
comments]. ╕
Source
╕
Fertility & Sterility. 59(4):826-35, 1993 Apr.
Title
Electroporation: a method for transferring genes into the gametes of
╕
zebrafish (Brachydanio rerio), channel catfish (Ictalurus punctatus),
and ╕
common carp (Cyprinus carpio).
╕
Source
╕
Molecular Marine Biology & Biotechnology. 1(4-5):301-8, 1992 Aug-Oct.
Title
Introducing foreign genes into fish eggs with electroporated sperm as
a ╕
carrier.
╕
Source
╕
Molecular Marine Biology & Biotechnology. 1(4-5):276-81, 1992
Aug-Oct.
Title
Electroporation of bovine spermatozoa to carry foreign DNA in oocytes.
╕
Source
╕
Molecular Reproduction & Development. 29(1):6-15, 1991 May.
Title
[The incorporation of macromolecules into the germ cells of male mice
via ╕
electroporation and dimethyl sulfoxide]. [Russian]
╕
Source
╕
Tsitologiia i Genetika. 24(3):3-7, 1990 May-Jun.
Hope this helps,
- Dima
