Alex Blais wrote:
> Hi,
>> I am trying to purify a recombinant protein fused to GST overproduced in
> E.coli. I have some problems during the purification steps, and I
> hypothesised that homodimers formation mediated by the GST moiety could
> be the cause of the problem.
Two questions:
How do you know there are dimers?
Why does that pose a problem?
Colin
