I am about to go for the expression and purification of new protein. So my
"simple" question is which expression systems are the best choice for this.
approx. 300 kD incl. glycosylation (important for epitops)
seems to be type I membrane protein
can be find in crude cell extracts and to a very small extent in supernatant
of cell culture
1. baculo system (I have a offer from novagen for their system)
2. mamalian cell culture transient and/or stable transfected with the
protein ( it is not expressed in i.e. COS cells)
Any hints and advices to my email address are well come.
Dr.Oliver Politz UKBF FU-Berlin Dermatology
Tel.: (+49)-30-8445 2765; FAX.: (+49)-30-8445 4262
email : politz at gmx.de