choice of expression system

Oliver Politz politz at medizin.fu-berlin.de
Tue Aug 31 03:03:50 EST 1999

I am about to go for the expression and purification of new protein. So my
"simple" question is which expression systems are the best choice for this.
The protein:
approx. 300 kD incl. glycosylation (important for epitops)
seems to be type I membrane protein
can be find in crude cell extracts and to a very small extent in supernatant
of cell culture

My ideas
    1. baculo system (I have a offer from novagen for their system)
    2. mamalian cell culture transient and/or stable transfected with the
protein ( it is not expressed in i.e. COS cells)

Any hints and advices to my email address are well come.
Thanks Oliver

Dr.Oliver Politz UKBF FU-Berlin Dermatology
Tel.: (+49)-30-8445 2765; FAX.: (+49)-30-8445 4262
email : politz at gmx.de

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