Human and electronic dynamic ranges for protein spot intensities

Douglas Kell dbk at aber.ac.uk
Mon Aug 30 04:36:22 EST 1999

1. Modern CCD cameras used in gel readers have a 16-bit dynamic range (or at
least save images as 16-bit tiff files)..
2. The human eye has a dynamic range for grey scale in a given pixel of not
more than 7 bits.
3. Conclusion: there is a lot more info in a gel than the eye can see, but
the processing we'd do on a computer etc to improve the image content or
dynamic range of an analysis does not  show up in a way that would a priori
persuade someone looking at a picture.

How do people deal with this? Can replies be cc'd to my mailbox as well as
this group?

Many thanks,
(Prof.) Douglas Kell
Inst. Biol. Sci., Cledwyhn Bldg,
UWA, Aberystwyth SY23 3DD, UK
dbk at aber.ac.uk    http://gepasi.dbs.aber.ac.uk

More information about the Proteins mailing list

Send comments to us at biosci-help [At] net.bio.net