It derivatises free sulfhydryl groups - you reduce cys-cys disulfide bonds,
react with iodoacetamide and they can't reform.
Standard procedure - look in any biochemistry text book.
Roger
In article <37c52585.486086 at news.univie.ac.at>,
a8803349.nospam at unet.univie.ac.at (Martin Offterdinger) wrote:
>What is the purpose in using iodoacetamide for protein isolation. (I
>found it in a protocol) Does it inhibit (thiol-) proteases? Could it
>also be used to neutralize DTT or BME?
>Any comments welcome!
>Martin
>Martin Offterdinger
>Internal Med.I,Dept. Oncology
>University of Vienna
>Austria
>E-Mail:a8803349.nospam at unet.univie.ac.at>(remove .nospam before mailing)
------------------------------------------------------------
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg, Vic. 3084
Australia.
Tel 61-3-94965463
Fax 61-3-94965436
Email Roger.Murphy at Ludwig.edu.au
