Hi Martin,
Iodoacetamide is usually used for peptide mapping purposes. It is added
after incubation of the protein with denaturants and reducing agents, in
order to covalently modify cysteine residues (to form S-carboxymethyl
cysteine) so that disulfide bonds can no longer form. Reducing agents are
then added to react any excess alkylating agent. These steps ensure that
the protein is completely unfolded prior to treatment with proteases.
Reno
On Thu, 26 Aug 1999, Martin Offterdinger wrote:
> What is the purpose in using iodoacetamide for protein isolation. (I
> found it in a protocol) Does it inhibit (thiol-) proteases? Could it
> also be used to neutralize DTT or BME?
> Any comments welcome!
> Martin
> Martin Offterdinger
> Internal Med.I,Dept. Oncology
> University of Vienna
> Austria
> E-Mail:a8803349.nospam at unet.univie.ac.at> (remove .nospam before mailing)
>>
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Reno T. Nguyen
Chesapeake Biological Laboratory /----\ /-----\ /
Univ. MD Center for Environmental Science / / / /
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