Dear all,
Is there a way of finding if a certain protein is likely to be short-lived
in a cell just by looking at the amino acid sequence? When making a
literature search, I have found many papers that mention an "N-end rule"
but I haven't been able to figure out what that is beyond knowing that a
Lys residue is needed for binding of ubiquitin. Besides, I got the
impresion that these signalling domains can be both at the N or the
C-terminus in a protein but I don't know if they are different. By the way,
I work in yeast, in case this makes a great difference in the way the
proteins are processed (as you can see, I haven't got much idea about all
these processes, sorry!)
Could someone point me to a good www site for analysing my protein sequence
and find if its turnover is likely to be rapid? Alternatively, anybody
knows of a review that deals with domains in a protein that signal it for
degradation?
I haven't got direct access to this newsgroup, so I'd appreciate if you
could send any answers to my mail address:
hernandez at pop.hort.purdue.edu
Thanks!
Agustin Hernandez
