I have made a recombinant of the substrate that a probable metalloprotinase
acts on.I have the choice to in vitro label this substrate with alpha P32.Now I
want to try different fractions of a particular tissue for enzyme clevage of
the substrate. I have no real hand experience with metalloprotinases. If you
are an expert please advice on a good protocol, buffer systems and possible
tips. I will appreciate your help.
You can e.mail me at
rlatif at smtplink.mssm.edu