hello!!
i'm using hydridoma supernatant to screen monoclonal antibody about
protein on a
membrane. since the amount of supernatant i can use is limited to a
small volume, i
used this apparatus named 'mini-protean2' from the bio-rad. but it has
been giving
me a problem of leakage between the wells. so i was wondering if anyone
could tell
me about similar kind of apparatus using a membrane to screen multiple
samples of
hydridoma supernatant or have any idea of eliminating the leakage
problem.
thank
you
joo jhong1 at uic.edu