Properties of proteins purified in imidazole

Marc Saric marc.saric at mpi-dortmund.mpg.de
Thu Aug 12 05:28:48 EST 1999

frank rozsa schrieb:
> 1. Does imidazole interfere with SDS-PAGE? If not, can I use the blot for
>    subsequent western blots?
No experience with Western Blotting, but it should work for normal
SDS-Gels (no problems here with a wider range of soluble proteins
expressed in E.coli).


Marc Saric

Max-Planck-Institut für molekulare Physiologie
Otto-Hahn-Strasse 11
44227 Dortmund

phone: +49 231 133 2168

More information about the Proteins mailing list

Send comments to us at biosci-help [At] net.bio.net