For native acrylamide gels (run in Tris-glycine buffer) we are looking for
dyes that would migrate significantly slower than bromophenolblue (BP) or
xylene cyanol (XC). Stained proteins are OK but not as desirable. We'd
like to exclude a lot of low MW material before we start collecting
fractions on a preparative electrophoresis cell. Size of the RNP
complexex to be isolated is ~150-350 kD
Thanks for any hints,
Juergen
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Juergen Brosius
Inst. f. Xp. Path./Molecular Neurobiology
University of Muenster
Von-Esmarch-Str. 56
D-48149 Muenster, Germany
Fax: +49 251 835 8512
Tel: +49 251 835 8511