IUBio

appratus for screening monoclonal antibodies???

Joo Yun Hong jhong1 at UIC.EDU
Thu Aug 5 11:15:54 EST 1999


hello!!

i'm using hydridoma supernatant to screen monoclonal antibody about
protein on a membrane. since the amount of supernatant i can use is
limited to a small volume, i used this appratus named  'multi-protean2'
from the bio-rad. but it has been giving me a problem of leakage
between  the wells. so i was wondering if anyone could  tell me about
similar kind of appratus using a membrane to screen mutiple samples of
hydridoma supernatant  or have  any idea of eliminating the leakage
problem.

                                                                   thank
you

joo         jhong1 at uic.edu





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