i'm using hydridoma supernatant to screen monoclonal antibody about
protein on a membrane. since the amount of supernatant i can use is
limited to a small volume, i used this appratus named 'multi-protean2'
from the bio-rad. but it has been giving me a problem of leakage
between the wells. so i was wondering if anyone could tell me about
similar kind of appratus using a membrane to screen mutiple samples of
hydridoma supernatant or have any idea of eliminating the leakage
joo jhong1 at uic.edu