Hi, I got a private reply from somebody whose newsserver didn't work:
From: "L. Guan" <lguan at is.icc.u-tokai.ac.jp>
To: Frank Fürst <ffrank at rz.uni-potsdam.de>
Subject: Re: Inclusion bodies
Date sent: Tue, 3 Aug 1999 19:12:02 +0900
Dear Frank,
Would you please let me know if inclusion body is lethal to cells? I
found
my mutants go to die if I use high concentration induction.
And, whether still have some native comformation protein out of
inclusion
body when inclusion body
forms? I restored the function of one of my mutants at 28 C. and also
have
partial function with low level induction. It seems the mutation do
not
attack the folding important structure.
Since I can not post this, sorry to bother you.
Thanks in advance!
Sincerely,
Lan
Department of Molecular life Science
Tokai University School of MedicineJapan
