I'm trying to visualize a crosslinked protein on a polyacrylamide gel, and
am having some difficulty with sharpness with the heavy bands. My
expected products have masses between 90 kD and 540 kD. I'm allowing the
90 kD monomer to run to the bottom of the gel. I've run a 4% 20 cm x 20
cm x 1.5 mm gel with a 4% stacking gel. Any suggestions as to how to
sharpen an expected 540 kD band?
University of Pennsylvania
Department of Chemistry
231 S. 34th St.
Philadelphia, PA 19104
okashlan at mail.sas.upenn.edu