> Please send /or post your favorite protease digestion portocols to me
I have recently used trypsin for detection of a post-translational
modification.
I used:
1% w/w trypsin in a 50 mM (NH4)(HCO3) pH 8.2
I stopped the reaction with a small amount of HCl and analysed directly
on a MALDI mass-spectrometer (by taken out samples I did a time course
analysis). This may of course not be the best way for you since your
protein is approx. 3 times larger than mine and you modification a bit
smaller but you could try. You could also try to purify the tryptic
peptides on a HPLC.
HTH
Kresten
>> Thanks in advance
>> Dirk
>
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Kresten Lindorff Larsen, Dept. Yeast Genetics
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