Hello,
I want to isolate nuclear proteins from whole tissue (specifically heart muscle) but without any
major disruption. I am used to making nuclear extracts from cell cultures by a modified version
of Dignam et al but have not been able to find a technique to isolate nuclear proteins from whole
tissue. In particular I want to isolate NF-kB which is very sensitive to stress and am therefore
looking for the most gentle method available for this.
I would be most grateful to anyone who has any ideas on the matter,
Rowena Johnson
Dr Rowena Johnson
Department of Cardiology,
Tenovus Building,
University of Wales
College of Medicine,
Heath Park,
Cardiff.
CF4 4XX
Phone: 01222 744518
