Recently, i have been dabbling in a bit of protein purification and on a
coomasie gel i have only a few proteins, one in particular is stronger than
the others. I wish to show that this stronger band is the protein
responsible for activity I am detecting in the fraction. Is it feasible to
simply elute this band from the SDS gel and assay this for activity, i.e.
is there a simple way of eluting the protein directly from the gel with a
good chance of renaturing the protein?
Thanks in anticipation
Karen Spink
E. mail pdxkgs at pdn1.gene.nottingham.ac.uk
