DE-52 Columns

[Leanne Allhouse]

I was once shown how to purify barnacle tnc proteins using DE-52 columns
using the buffer 6M urea, 50mM Tris-HCl, 1mM EDTA,1mM DTT but was
wondering if anyone can tell me why it is important to measure the
conductivity of the column buffer, loaded sample and eluted fractions?

Thanks
Leanne