Hi group,
I'm doing Western to study genes transformed into yeast. Funny thing was that
I got the same banding patterns in transformed and untransformed strains. So
the other day I probed my yeast lysate with secondary antibodies only. What do
you know, I have the same bands! I tried a few secondary Abs, including
monoclonal mouse anti-Rabbit IgG! Guess I'm going to do immunoprecipitation
before Western next time, but I'm wondering if anybody has any comment on this?
Thanks,
Red Lowe
