François Mallet wrote:
> We try to purify a protein from human urine. The goal of our our work is
> to sequence this protein that have an biological activity. However, after
> multiple steps of purification, and although the specific activity of the
> enriched protein increased, this protein remains contaminated with large
> amounts of albumin. Thus we are unable to sequence it.
>> Could you give me some advices on the way to discard the albumin.
>> Thank you
>> Francois mallet
I would first try affinity chromatography on immobilised Cibacron Blue dye
(e.g. Blue Sepharose), it binds albumin very strongly.