We try to purify a protein from human urine. The goal of our our work is
to sequence this protein that have an biological activity. However, after
multiple steps of purification, and although the specific activity of the
enriched protein increased, this protein remains contaminated with large
amounts of albumin. Thus we are unable to sequence it.
Could you give me some advices on the way to discard the albumin.
Thank you
Francois mallet