IUBio

His-tagged protein and SDS-PAGE migration

Dr E. Buxbaum EB15 at le.ac.uk
Fri Mar 27 15:55:43 EST 1998


Darren Tyson wrote:

> (although I've never seen a protein migrate faster than expected
> on SDS-PAGE... how about anyone else?)

That can happen with very hydrophobic proteins (for example
transmembrane proteins), which bind more SDS than they should for their
size. A typical example would be the Na/K-ATPase alpha subunit, which
has a MW of 114 (from sequence), but runs at about 80 kDa.



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