Fei WANG wrote:
> I plan to purify u-PA and have a problem to solve. In the last step, I
> should to concentrate the u-PA, does anyone know the best way to concentrate
> with less-inactivation and with less-convert from one-chain form to
> two-chain form. Please help me.
Hi Fei WANG
wot is u-PA?
if it is a protein the dimerisation might be concentration dependent anyways?
jez
--
. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-
|\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X||
||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|
-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `
Jeremy Murray MAIL: bmbjmm at bmb.leeds.ac.uk
Biochemistry & Molecular Biology TEL: +44 113 233 2591
Leeds University, LEEDS LS2 9JT FAX: +44 113 233 3167
. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-
|\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X||
||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|
-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `
