nonsense. his-tag won't by itself cause nothing more than an increase
in MW of 600.
but, being a recombinant protein, all kinds of screwy things can happen.
your question is too general because every protein would have an
emperical answer.
Jim Bassuk
U Washington
Seattle
On Tue, 10 Mar 1998 ct133 at columbia.edu wrote:
> Hi,
>> Does anybody know whether his-tagged protein expressed from pRSET
> vector (Invitrogen) can migrate aberrantly on SDS-PAGE?
>> Thanks,
>> C. Tunyaplin
>>
