Dear all
I have expressed my peptide with fusion to Schistosoma japonicum GST to
its N-terminal.
The protein was in insoluble fraction. I have tried to use lower
temperature for the expression. Now,I try to refold it,so far it is
unsuccessful. I tried unfolding the protein in 6M guanidine HCl, then
stepwise dialysis from 6M guanidine HCl to 4,2,1,0.5 and 0 M guanidine
HCl respectively. 4-6 hr after dialysis against 0M guanidine HCl, I can
observed some aggration. All processes were done at 4-6 'C.
Anyone have suggestion in refolding of GST fusion protein. I do not use
detergent ,because I may interfere my experiment. Any suggestions and
comments are welcome.
TIA.
Jongrak
--
-----------------------------------Mr. Jongrak Kittiworakarn
g3937506 at student.mahidol.ac.th
Inst. of Science and Technology for Research and Development
Mahidol University (Salaya Campus)
Salaya, Nokornprathom, THAILAND.
Tel. 001-66-2-441-9003-7 ext.1277,1278,1279
Fax. 001-66-2-441-9906, 001-66-2-441-1013
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