Who has experiences with enterokinase?
I want to use it for cleaving a fusion protein and have some questions:
Is enterokinase really specific for DDDDK?
Does enterokinase modifiy the new amino terminus after cleavage?
Are there any "special conditions" for the cleavage? E.g. temperature,
molar ratio, ...
Thanks a lot.
Peter
*****
_________________________________________________________
DO YOU YAHOO!?
Get your free @yahoo.com address at http://mail.yahoo.com