Hello netters
E. coli expression does not seem to work for my huge protein. So while I am
playing with E. coli vectors, I plan to start with yeast expression. Who
has experience with yeast? Can you suggest some vectors with strong
promoters? What are the important points in yeast transformation? I know
that is the thoughest part usually. I am a beginner so every suggestion
will be appreciated. Thank you all.
Benan