IUBio

bandshift in agarose gel

Markus Zettl markus.zettl at kfunigraz.ac.at
Thu Jan 29 03:07:53 EST 1998


Hi all,
I wanted to carry out a modified bandshift where I mix DNA, and two
proteins.
If an interaction of these two proteins exists the DNA-protein-protein
complex has about 100 kDa. 
Is it possible for this complex to an 2-3% agarose gel?
Do you something about Protein-protein interactions in such asystem?



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